Figure 1: The McCord Formulation accelerates cell migration and improves wound healing in human microvascular endothelial cells (HMEC-1). Cells were pretreated with the McCord formulation for 24 hours, then scratched and imaged regularly until complete wound closure. The time taken for 50% wound closure was recorded.
Table 1: Time (hours) required for 50% wound closure following treatment with McCord Formulation, individual components, and McCord formulation without specific components. The synergistic effect of all the components in the formulation resulted in shortest wound closure time (best wound healing effect). Removal of hydroxytyrosol from the McCord Formulation resulted in the longest wound closure time and therefore greatest decrease in effectiveness of the McCord Formulation.
Figure 2: The McCord Formulation restores microvascular endothelial (HMEC) cell migration and wound closure in the Doxorubicin and high glucose model of impaired wound healing.
Figure 3: Olivamine improved cell migration and wound healing in human umbilical vein endothelial cells (HUVEC) pre-treated with high glucose (30 mM) for 72 hours. Hydroxytyrosol also improved wound healing compared to untreated cells.
Figure 4: Olivamine improves angiogenesis (vascular tube formation) in human umbilical vein endothelial cells (HUVEC). Mean ± standard deviations from a single experiment performed in triplicate are shown; total of 2 independent experiments tested.
Figure 5: Olivamine improves the antioxidant capacity of normal PBMC. Olivamine also restored the depleted antioxidant capacity induced by stress with high glucose by 70% and doxorubicin by 82%. Mean ± standard deviations from an experiment performed in triplicate are shown; 2 independent experiments were performed.